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Crystal structure
PDB 5CTS — chicken citrate synthase with oxaloacetate + carboxymethyl-CoA (citryl-CoA analog), Remington S et al., J Mol Biol 1982, PMID:7120407.
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Mechanism — ternary complexes
Karpusas M, Holland D, Remington SJ, Biochemistry 1991, PMID:2043640 — malate ternary complexes define the substrate position for the Claisen condensation; His274 acts as the general base that forms the acetyl-CoA enolate.
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Sequence
Chicken CS UniProt P23007 · human mitochondrial CS UniProt O75390 · NCBI Gene 1431 (human CS)
› Backbones and ligand poses are taken directly from the listed PDB entries,
superposed into one common frame. Where no crystalline state exists for a step
(e.g., acetyl-CoA approach, water attack), the pose is drawn schematically and
labeled as such in the narration.