-
Crystal structure
PDB 5UPP — human fumarate hydratase (apo), Ajalla Aleixo MA et al., FEBS J 2019, PMID:30761759. The RTHTQ loop (Arg233-Thr234-His235-Thr236-Gln237) is the catalytic loop.
-
Mechanism — anti-addition
Bekker M, et al., FEBS J 2018, PMID:29573314 — stereospecific trans (anti) hydration of fumarate to L-malate with His235 as general acid/base; a reversible lyase reaction.
-
Sequence
Human FH (mitochondrial) UniProt P07954 · NCBI Gene 2271 · fumarase deficiency / tumor-suppressor relevance documented at OMIM 136850
› Backbones and catalytic loops are taken directly from the listed PDB entries,
superposed into one common frame. The 5UPP entry is apo (no substrate); the
fumarate/water/malate poses are drawn schematically and labeled as such in the narration.