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Human structure
PDB 1JLH — human PGI (Cordeiro AT et al., BBA 2003, PMID:12573240); sugar-phosphate-analog complex 1IRI (Tanaka N et al., J Mol Biol 2002, PMID:12054796).
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① Ring opening — Glu357 shuttle
[schematic] Glu357 (general base) opens the ring by abstracting the C1-OH proton while His388 donates H⁺ to the ring oxygen — converting glucopyranose to the open-chain aldehyde.
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② cis-enediol(ate) — proton swap
[schematic] The carbonyl moves C1→C2 through a cis-enediolate stabilized by His388/Lys518; Glu357 abstracts C2-H, His388 returns H to C1 (glucose↔fructose interconversion).
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③ Ring reclosure — F6P out
[schematic] The aldehyde recloses to the furanose and F6P exits toward PFK-1; fully reversible, near-equilibrium step.
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Sequence
DNA/gene NCBI Gene 2821 · protein UniProt P06744
› Backbones and ligand poses are taken directly from the listed PDB entries,
superposed into one common frame. Where no crystalline state exists for a step
(e.g., Michaelis geometry), the pose is drawn schematically using PubChem 3D
geometries and is labeled as such in the narration.